Cell

Part:BBa_K4832003:Design

Designed by: Zhang Guiming   Group: iGEM23_HUBU-WUHAN-CHINA   (2023-10-09)


P.chlororaphis KC-P


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When knocking out RNase III gene，the test for whether the strain has knocked out RNase III gene should be meticulous, and attention should be paid to false positives and other situations



Source

This part comes from the wild Pseudomonas chlororaphis.We amplified and sequenced the RNase III sequence of Pseudomonas chlororaphis based on the reported sequences of Pseudomonas and ultimately determined the RNase III sequence of Pseudomonas chlororaphis. Based on this, genetic operations were carried out

References